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Sample Design: Redfish Lake Sockeye Salmon latent mortality study
  • Version History: v1.0 Draft (9/16/2021)
This is an abbreviated view of sample design Redfish Lake Sockeye Salmon latent mortality study. To view this sample design in full you need to be logged in.
Basics

The details of this Sample Design, including all the parameters used to generate it, are included below. Sample designs must belong to a Study Plan.

Description

Hatchery and release site: Starting six weeks from acclimation transport, brood year 2020-2022 Springfield Fish Hatchery reared smolts will be monitored for smolt development and stress physiology every two weeks. Smolt development and stress physiology will be monitored at Sawtooth Fish Hatchery weekly during acclimation. At each sample point we will collect 25 individuals and measure condition factor, cortisol, and hematocrit, blood lactate, Na+ and Cl- levels, gill Na+/K+- ATPase activity. Differences in condition factor, cortisol, and hematocrit, blood lactate, Na+ and Cl- levels, and gill Na+/K+- ATPase activity among rearing treatments (8, 10, 12, and 14 fpp), and across time, will be tested.

Morbidity and mortality will be monitored in smolts held for 14 days post-release in Redfish Lake Creek. We will perform a seawater challenge at the end of this 14-day holding period by exposing smolts to artificial seawater at 33 parts per thousand. Mortality rates will be calculated across 24 hours of seawater exposure.

        • Migratory corridor: Springfield Fish Hatchery reared smolts will be sampled at the Lower Granite Dam juvenile bypass. We will measure gill Na+/K+- ATPase activity to monitor smolt development during migration.

 

Study Design — Hatchery and release site

 

Mid-March

Late-March

Mid-April (at transfer to SAW)

Springfield Hatchery

n=25 fish

-pathology sample (disease)

-Condition Factor

-Cortisol

-Lactate

-Hematocrit

-Blood Na+ and Cl-

-Gill ATPase

n=25 fish

-pathology sample (disease)

-Condition Factor

-Cortisol

-Lactate

-Hematocrit

-Blood Na+ and Cl--Gill ATPase

n=25 fish

-pathology sample (disease)

-Condition Factor

-Cortisol

-Lactate

-Hematocrit

-Blood Na+ and Cl-

-Gill ATPase

 

 

Mid-April (after transfer to SAW)

Late April (before release to RFLC)

May (at release to RFLC)

Sawtooth Hatchery

n=25 fish

-pathology sample (disease)

-Condition Factor

-Cortisol

-Lactate

-Hematocrit

-Blood Na+ and Cl-

-Gill ATPase

n=25 fish

-pathology sample (disease)

-Condition Factor

-Cortisol

-Lactate

-Hematocrit

-Blood Na+ and Cl-

-Gill ATPase

n=25 fish

-pathology sample (disease)

-Condition Factor

-Cortisol

-Lactate

-Hematocrit

-Blood Na+ and Cl-

-Gill ATPase

 

 

May (at release to RFLC)

May (1 week after release)

May (2 weeks after release)

Redfish Lake Creek

-Place 100-150 fish in live box at Creek during release

n=25 fish from live box

-pathology sample (disease)

-Condition Factor

-Cortisol

-Lactate

-Hematocrit

-Blood Na+ and Cl-

-Gill ATPase

n=25 fish from live box

-pathology sample (disease)

-Condition Factor

-Cortisol

-Lactate

-Hematocrit

-Blood Na+ and Cl-

-Gill ATPase

 

Study Design — Migratory corridor

 

May

Lower Granite Dam Juvenile Bypass

n=25 ad clipped

-pathology sample (disease)

-Condition Factor

-Gill ATPase

-Injury assessment

 

 

 

Start Year

2022

End Year

2022

Study Plan

Redfish Lake Sockeye Salmon latent mortality study v1.0

Data Repositories

Photos

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Documents

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Area of Inference

<none>

AOI Notes

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