Environmental DNA (eDNA) samples will be collected opportunitstically, as needed to detect the presence/absence or range of a target species as a direct result of a management action (e.g., non native species suppression/eradication, native species reintroduction), proposed management action, or suspected introduction of an invasive species.  The quantity and distribution of samples will depend on the question but follow protocols provided in the literature. Samples can be taken during any calendar months when freezing tempertatures are not present.

Examples of potential sampling include: presence/absence sampling for Brook Trout or Westslope Cutthroat Trout at the suspected upper extent of distribution and or every 200-300 m of stream; sampling of lakes and rivers along shorelines or at tributary mouths for suspected invasion of non-native fish species (e.g., Northern Pike, Lake Trout, Smallmouth Bass, etc). 

Samples will be taken as opportunities arise or are necessitated by projects and/or new information or management direction. Examples may include 1) determining presence/absence of target native or non-native species within a management projects, such as non-native species suppression or eradication; 2) evaluation of a suspected introduction of an invasive species; 3) determining the spatial extent of a target species within a watershed (i.e., upper and lower distribution). Additional sampling strategies not listed here may be employed using this technique or method.